Enzyme Catalase Lab- Toure Jones

Video




Example Data
This is example data that could have been recorded in an experiment

This is a graph that would have showed the initial reaction rate of the enzyme, then its decrease due to increased saturation with substrate, causing it to plateau.



Example Calculations
You must calculate: The amount of KMnO4 used, and amount of H2O2 used, and baseline

To Calculate the Amount of KMnO4 Used
Equation: (Baseline- Final)

For 10 seconds: (4.0-2.2)= 2.8 mL

Example Problem: Baseline-6mL/ Final Volume of KMnO4- 2.3

(6-2.3)= 3.7 mL used

To Calculate the Amount of H2O2 Used
Equation: (Baseline- Amount of KMnO4 used)

For 10 seconds: (4.0-2.8)= 1.2 mL H2O2 used

Example Problem: Baseline- 6mL/ Amount of KMnO4 Used- 5.9

(6-5.9)= 0.1 mL used

To Calculate Baseline
Equation: (Final volume- Initial volume)

For 10 seconds: (5- 2.2)= 2.8 mL is the baseline

Example Problem: Final volume- 6 mL/ Initial volume- 4 mL

(6-4)= 2 mL is the baseline

Quizlet
Link: https://quizlet.com/82111704/enzyme-catalase-lab-questions-flash-cards/

Connections
This lab connects to:
 * The function of enzymes in transcription: DNA polymerase is an enzyme that participates in transcription. This enzyme adds nucleotide bases, constructing an RNA strand, copying from a DNA strand.
 * The affect environmental factors have on enzymes: Environmental factors such as pH and temperature can affect the reaction rate of certain reactions by denaturing the enzymes themselves. This will slow the reaction down to where it would be without the enzyme.
 * The affect of substrate on the enzyme reaction rate; An enzyme has active sites to which substrate can bind to, yet only a certain amount. If the enzyme becomes saturated, then the maximum amount of substrate the enzyme can hold is reached, and the enzyme's rate of reaction will no longer increase, and will plateau.